Student Web quest: Gel Electrophoresis Source: Genetic Science Learning Center. Why do you need to place a nylon membrane over the gel? The aragose gel is to hard to work with. Since these DNA fragments are too large to separate by conventional agarose gel electrophoresis, the use of an alternating voltage gradient system known as pulsed-field gel electrophoresis was developed. You will perform agarose gel electrophoresis on the PCR products generated during the previous lab exercise, as well as the restriction digest reactions generated today. Forensic DNA Fingerprinting Lab from Bio-Rad; Candy Dye Analysis using Gel Electrophoresis from Bio-Rad; All participants receive: Access to free lab materials through the Baxter Box Loaner Program. The molecules being separated are usually DNA, RNA, or protein molecules. Once it goes into the Thermal Cycler it will be heated so it must be cold first. Animation Click here for Animation. VIRTUAL LAB. Gel_Electrophoresis_Webquest_2018 (1). Starch gel electrophoresis, which makes use of potato starch granules, is something of a relic. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. Objective: Students will learn the steps of DNA fingerprinting by creating a fingerprint in a virtual lab. Protein Gel Electrophoresis Animals Evolution Genetics High School Inquiry/Scientific Method Molecular Biology. These processes use agarose get to isolate and study proteins and DNA. The electrophoresis reagents segment accounted for the largest share of the market in 2019. Emblaser 2 Laser Cutter and Engraver. Investigators use a process called gel electrophoresis to separate these. This lab can be easily shared with students through Google Classroom or the use of a Google Drive. California Science Center Link. In this part of the laboratory, you will use gel electrophoresis to separate samples of DNA that have been digested by restriction enzymes. Your answers must be submitted by 9:00 am on the day of the lab. polyacrylamide gel, stain the gel, and analyze the resulting bands of protein on the gel to estimate the molecular weight of each protein. Effect of Inhibitors on Enzyme Activity. In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. Background: As the newest and least experienced student in a biochemistry research lab, you spend your first few weeks washing glassware and labeling test tubes. An interactive project to explore DNA. DNA Extraction Virtual Lab. Gel Electrophoresis Lab. 10805 Vista Sorrento Parkway Suite 200 San Diego, CA, 92121 Directions Telephone: 858-550-0800 Fax: 858-550-0255 Email: [email protected] null BioSurplus. "Once you've done that, you can use chromatography and electrophoresis to analyze both the plasmid DNA and the proteins produced by the transformed cells. Title: Biotechnology Webquest Gel Electrophoresis Answer Key Keywords: Biotechnology Webquest Gel Electrophoresis Answer Key Created Date: 9/5/2014 4:59:33 PM. Charged molecules move through a gel when an electric current is passed across it. DNA Learning Center. Related posts of "Virtual Gel Electrophoresis Lab Worksheet" Community Helpers Police Officer Worksheet Previous to discussing Community Helpers Police Officer Worksheet, make sure you are aware that Training can be all of our key to a more rewarding down the road, and also discovering doesn't just stop once the education bell rings. Dissertation Abstract. This product is a student handout I have created based on an existing virtual lab on gel electrophoresis. WOW BioLab- Gel Electrophoresis Name: Go to “classzone. This will allow you to visualize your results and capture the image files for further analysis. Virtual Gel Electrophoresis Lab Worksheet having Beneficial Subjects. DNA Extraction. Questions: 1. You will find a unique blend of products for Arts & Crafts, Education, Healthcare, Agriculture, and more!. PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. Sequencing is the process by which scientists learn the exact order of bases in a length of DNA. “ Polyacrylamide Gel Electrophoresis. These are collection of lab activities developed from the Virtual Courseware Project at Cal State University-Los Angeles. The lab notebook can be found under the VIRTUAL LAB NOTEBOOK tab. What does a restriction enzyme do to DNA? 5. We will use it for analyzing gel electrophoresis experiments. Setting up the SDS-PAGE Apparatus: Images from College of Charleston's Virtual Molecular Biology LabBook. Activity Summary Hands-on labs teaching students about biotechnology can be expensive and tedious for teachers to prepare. During the running and staining of your gel (steps 9 and 11), perform Part II of this lab, "Extraction of Mitochondria", which will be needed for next week's lab. Get Your Custom Essay on Bacterial Transformation Lab Report Just from $13,9/Page Get custom paper The ara control region regulates GFP expression by the addition of arabinose, so the GFP gene can be turned on and off by including or omitting arabinose from the culture medium. Forensic DNA. What are the 5 steps for. Kits and materials for educators by educators. What is Step 3? 8. molecules migrated from the well in gel depends on the size and charge of the molecule. gel electrophoresis and determination of genotype through gel electrophoresis. 10/2/2018 0 Comments Tuesday - 10/2. Virtual Lab - Electrophoresis This process is similar to separating molecules by chromatography, which you did with marker ink and with leaf pigments. Running the gel: Note : Before running the gel make sure that the gel, gel apparatus and samples are ready. DNA isolation-->restriction enzyme digestion-->gel electrophoresis. What is Step 4? 9. 2 Quiz Study Guide - QUIZ ON TUESDAY!!!. Agarose gel electrophoresis separates DNA fragments according to size (see Figure 2). Gel Electrophoresis Virtual Lab Posted on April 5, 2011 by heymisterlee A well-designed “virtual lab” from the Genetic Science Learning Center from the University of Utah. For example, Southern Blotting could be used to. The first process done is the two-dimensional gel electrophoresis or 2D page, which is commonly used for proteins. In Gel Electrophoresis Proteins and nucleic acids are electrophoresed inside a matrix or "gel". Essay about Gel Electrophoresis. 7 May 2020. Photo Documentation System d. Lab report that discusses the antigen-antibody precipitation reaction using the Ouchterlony Double Diffusion Technique. You will find a unique blend of products for Arts & Crafts, Education, Healthcare, Agriculture, and more!. Lab 10 gel electrophoresisworksheet x electrophoresis 3 pages virtual homework assignment 1 doc. Answer the questions below as you perform the lab. 2 Gel Carrier Sides - 33⁄4" by 1⁄2" by 1⁄2" thick 1 Gel Carrier Base - 33⁄4" by 33⁄4" by 3⁄4" thick Cut a slot 3⁄16" from one end of each Gel Carrier Side. , length in base pairs) for visualization and purification. Extensive intercompany studies demonstrate. What is gel electrophoresis, you might ask. Essay about Gel Electrophoresis. Our crime laboratory is very much like a 'typical' police lab, except the victim is an animal. The VEG has become a popular device for the laboratory and its popularity is growing with the fact that it is a fully functional unit with additional features that were not available in the past. However, a postelectrophoresis incubation of the gel at 4°C, to allow ethidium ions to migrate to the DNA, produced faint bands (Figure 2 B). Mini-Practical: PCR & Gel Electrophoresis Virtual Lab. Group Size: For 10 lab groupsTime Required:PCR Setup - 10 minutesPCR - 30 minutesElectrophoresis - 30 minutes Staining - 5 minutes to overnightKit Includes: Instructions, PCR EdvoBeads. Be sure to use the comb with thicker, wider teeth to ensure that the resulting wells can hold a. Due to the fact we wish to offer all you need in a single genuine as well as reputable supply, most people present valuable information about numerous subject matter and also topics. An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding and Enhancement Naima Kaabouch1, Member, IEEE, Richard R. To understand how the process works, one must first learn the gel electrophoresis definition. DNA sequencing using dideoxynucleotides and electrophoresis. The percentage of gel you require corresponds with the MW of your target protein. Tens of 3D simulations for the major experiments of Biology, Chemistry & Physics. There's a simple set up with consistent results. Mark Stanback. The Amgen Biotech Experience is an international education program funded by the Amgen Foundation. For use with 96-well plates, including QIAamp 96 plates and RNeasy 96 plates. A partial list can be found here. doc, DjVu, txt forms. Students will be able to show their knowledge of the lab protocol as they perform the lab. Student Worksheet Virtual Lab. Ppt Biotechnology Genetic Engineering Powerpoint"> Full Template. It consists of just a few kinds of atoms: carbon, … Continue reading "DNA Replication Lab". and gel electrophoresis to perform genotyping in order to identify the gene that causes double muscling in cattle. Each student will have access to their own computer to experience the virtual lab. DNA Extraction. Run the gel for 15 minutes. Directions: For this mini-practical you are going to be doing two online simulations: one of PCR (polymerase chain reaction) and one of gel electrophoresis. Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size. The molecules will move faster or slower based on their size and electric charge. Flinn Blended Learning Labs for Chem: Manipulating Equilibria to Determine Sulfites in Grape Juice. Heres your chance to try it yourself. To start the process, the DNA segment of interest is heated to disassociate the. Navigation. Chapter 12 Dna Rna Answers Guided Reading Study Work, Guided Reading Activity 10. After using the Electrophoresis to process some DNA, what was the estimated length, in base pairs, for each band in your DNA sample? (You will find this after you go through the electrophoresis virtual lab. The gel is cast in the shape of a thin slab, with wells for loading the sample. Unformatted text preview: Noah Butler Student Web quest: Gel Electrophoresis Source: Genetic Science Learning Center. This entails electrophoresis, blood typing, polygraph, fingerprinting and more. Before we complete that lab I want you to analyze DNA from another crime and evaluate the evidence. A vLAB focusing on Western Blotting was developed and implemented in a 3rd year undergraduate Pathology course for science students to facilitate. Gel electrophoresis, then, refers to the technique in which molecules are forced across a gel by an electrical current. This activity takes approximately 3 hours to complete with time for other activities between various steps. After this gel is finished, we will visualize it (see step 3 below). DNA fragments are separated according to their size. DNA gel Electrophoresis. After electrophoresis, DGGE gels were stained with SYBR ® Gold Nucleic Acid Gel Stain (Molecular Probes, Eugene, OR) (stock solution diluted 1:10 000 in 0. The gel is then subjected to an electric field, which draws the negatively charged DNA across it. What is CRISPR? Bozeman Science. Lab 10 gel electrophoresisworksheet x electrophoresis 3 pages virtual homework assignment 1 doc. I chose to look at my mixture by using 1d and 2d polyacrylamide gel electrophoresis (PAGE) and an immunoblot. DNA RESTRICTION DIGEST AND GEL ELECTROPHORESIS: A VIRTUAL LAB by bcitstudents – Click here to launch virtual lab – (Screenshot #1) This is the virtual version of the UBC Advanced Molecular Biology Laboratory’s experimental kit #2 (see Restriction Digest of Lambda DNA and Gel Electrophoresis for details) which features a common and important molecular technique used in laboratories to. Along the way, we'll learn about techniques (such as polymerase chain reactions and gel electrophoresis) that forensic scientists use in DNA fingerprinting. Webmix users: 1 Users. html Answer. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis. The separation of these molecules is achieved by placing them in a gel made up of small pores and setting an electric field across the gel. The DNA result was submitted to the CODIS DNA databank which forwarded the name John Doe as the suspect. Virtual Demos; Molecular Biology Methods. Lab 10 gel electrophoresisworksheet x electrophoresis 3 pages virtual homework assignment 1 doc. In Gel Electrophoresis Proteins and nucleic acids are electrophoresed inside a matrix or "gel". Gel Electrophoresis Drew Linarelli Honors Biology 17 May 2016 Period 9. The virtual lab can be found at the following link: Gel Electrophoresis Virtual Lab The student handout was created specifically for Distance Learning and was formatted using Google Docs. Suggested skill level: Intended for any student seeking basic familiarity with DNA gel electrophoresis and micropipetting, from middle school to college. In our lab, we will have two groups run gels on the same apparatus. Gel Electrophoresis. Follow the steps below to prepare genomic DNA for agarose electrophoresis. PCR is the amplification of a small amount of DNA into a larger amount. Chemistry 221. Purpose: To investigate the process of Gel Electrophoresis in DNA fingerprinting. Unformatted text preview: Noah Butler Student Web quest: Gel Electrophoresis Source: Genetic Science Learning Center. Gel Electrophoresis Virtual Utah Lab Answer Key Author: symsys03. How do the DNA strands move through the gel? 4. The lab uses their Pulsed-field Gel Electrophoresis (PFGE) equipment to DNA fingerprint bacterial isolates. RNA: transcription; compare to. This process uses electricity to separate DNA fragments by size as they migrate through a gel matrix. Temperature Effect. Gel Electrophoresis Virtual Lab. In this set of activities students will learn about DNA extraction, gel electrophoresis, PCR and cloning through online interactive virtual labs. Gel electrophoresis is a procedure used to separate biological molecules by size. PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. Gel Electrophoresis Simulation. PAGE is a powerful analytical technique having numerous applications in modern biology. It is based on the principle of migration of charged proteins in an electric field with separation occurring on the basis of net charge and size of the protein. In this lab students will model the procedure of setting up the gel, loading the DNA samples, assembling the gel apparatus, hooking up the electric current, and finally analyzing the results. Create a gel electrophoresis lab using the following virtual lab:. Instructional Material. Answer all questions in the lab notebook to complete the lab. Post-Doctoral Project Summary. BIO 2960 Lab: DNA Structure Computer Lab. Even though this lab presents a different application for gel electrophoresis, the lab helps you to understand how this. Fast Blast Staining Facility g. For use with 96-well plates, including QIAamp 96 plates and RNeasy 96 plates. Sequencing is the process by which scientists learn the exact order of bases in a length of DNA. "Gel Electrophoresis Virtual Lab. DNA Extraction Virtual Lab, PCR Virtual. Click the Explore button to learn about the lab items available for your investigation. It is mostly liquid but does give some inert support – there is an average pore size of interlaced strands. In gel electrophoresis, a sample of DNA is first "loaded" onto a slab of agarose gel (literally pipetted into small wells at one end of the slab). Select "Gel Electrophoresis" from the list and start the virtual lab. Journal Club. Gel Electrophoresis Adventure Intro The final goal of this lab was to successfully measure the size of different samples of DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. Agarose gel electrophoresis is a method of choice for large molecule separation over 1 million Da. What is gel electrophoresis, you might ask. DNA Extraction. In our lesson, we discussed using gel electrophoresis for nanotechnology, specifically determining if the PEG molecule has been attached to the quantum dot. Sequencing is the process by which scientists learn the exact order of bases in a length of DNA. In this lab, students will learn to use image processing techniques to analyze and quantify image data from wet lab experiments such as those in cell biology, biochemistry, molecular biology and immunology laboratories. SDS-PAGE stands for Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis. Carousel Previous Carousel Next. What is electrophoresis used for? 2. Virtual Dissection. What makes the DNA move? 4. Gel Electrophoresis Webquest DNA Fingerprinting "Virtual Lab" www. The molecules will move faster or slower based on their size and electric charge. After removing the enzymes, why do you spin the sample in the centrifuge?. McCoy’s Syllabus; Online Textbook Link; Unit 1 – Introduction, The Crime Scene and Physical Evidence; Unit 2A Fingerprints, Hair & Fibers; Unit 2B Questioned Docs, Glass, and Soil; Unit 3 Medicolegal Death; Unit 4 – DNA, Blood & Spatter, and Drugs. What is CRISPR? Bozeman Science. 12 - Virtual PCR lab, virtual gel electrophoresis lab, web-based activities Feb. Starch gel electrophoresis, which makes use of potato starch granules, is something of a relic. Education Center - K-12 Lessons and Laboratories - Classroom Activities in Plant Biotechnology: Activity 2 - Gel Electrophoresis of DyesIntroduction This experiment will teach students how to prepare and load an electrophoresis gel. Measurements in Biology. Gel Electrophoresis Virtual Lab Worksheet Read Book Gel Electrophoresis Virtual Lab Worksheet Gel Electrophoresis Virtual Lab Worksheet If you ally need such a referred gel electrophoresis virtual lab worksheet book that will have enough money you worth, get the agreed best seller from us … Gel Electrophoresis Virtual Lab. Gel Electrophoresis Virtual Lab. From a Computer with the Program: Select Gel Electrophoresis from the list of labs. The lab notebook can be found under the VIRTUAL LAB NOTEBOOK tab. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Once it goes into the Thermal Cycler it will be heated so it must be cold first. ID: 15921; Source: DNAi. Today, we'll be talking about gel electrophoresis. These are collection of lab activities developed from the Virtual Courseware Project at Cal State University-Los Angeles. Which strands move father? Why do you think they are able to do this? 5. polyacrylamide gel, stain the gel, and analyze the resulting bands of protein on the gel to estimate the molecular weight of each protein. Unformatted text preview: Noah Butler Student Web quest: Gel Electrophoresis Source: Genetic Science Learning Center. In this lab, students prepare and analyze a virtual bacterial DNA sample. Title: Gel Electrophoresis Virtual Lab Author: FCHS Last modified by: FCHS Created Date: 3/6/2008 3:51:00 PM Company: PSD Other titles: Gel Electrophoresis Virtual Lab. AP Biology 12 Molecular Biology Lab 6B: Virtual Electrophoresis Learning Intentions o How does gel electrophoresis separate DNA fragments? o How can you use a standard curve to determine the size of unknown DNA fragments?. Once it has cooled the comb is removed. – MLT to MLS Overview. 1, the stained protein bands representing albumin, α 1 - , α 2 - and β-globulins are easily demonstrated. This worksheet is for use with the bacterial identification lab, and gives students instructions and questions related to the lab. You will find a unique blend of products for Arts & Crafts, Education, Healthcare, Agriculture, and more!. In this interactive activity from the Dolan DNA Learning Center, learn about gel electrophoresis, the standard technique that makes comparative analysis of DNA samples possible. Even though this lab presents a different. Title: WOW BioLab- Gel Electrophoresis Author: twolfe Last modified by: twolfe Created Date: 3/22/2013 5:28:00 PM Company: Amador Valley High School. The purpose of this assignment is to give you a better understanding of biotechnology – technology using living organisms for a specific purpose or product. Our western blot protocol includes solutions and reagents, procedure, and useful links to guide you through your experiment. if you like this animation. In gel electrophoresis, a sample of DNA is first "loaded" onto a slab of agarose gel (literally pipetted into small wells at one end of the slab). Directions: For this mini-practical you are going to be doing two online simulations: one of PCR (polymerase chain reaction) and one of gel electrophoresis. Related posts of "Virtual Gel Electrophoresis Lab Worksheet" Community Helpers Police Officer Worksheet Previous to discussing Community Helpers Police Officer Worksheet, make sure you are aware that Training can be all of our key to a more rewarding down the road, and also discovering doesn't just stop once the education bell rings. The presence of M proteins can be a sign of a type of cancer called myeloma, or multiple. 11/20/18 Gel Electrophoresis. The DNA you have extracted is genomic, meaning that you have the entire collection of DNA from each cell. In agarose gel electrophoresis, a purified, large molecular weight polysaccharide is used as the medium; this is commonly used for large DNA molecules. Why is "gel" used? 3. This exercise is a simulation of human karyotyping using digital images of chromosomes from actual human genetic studies. Purpose: This activity is meant to learn and understand how protein can be isolated from DNA fragments through gel electrophoresis technique. Learning Objectives: Upon completion of this activity, students will: 1. What is the material of the gel similar to? A sponge made of jell-o 3. The activity is completed with an electrophoresis to test for Huntington's disease in the patients. To start the process, the DNA segment of interest is heated to disassociate the. Designer tips, volume 2: Common color mistakes and the 60-30-10 rule; 6 May 2020. Then you will compare fragments of unknown size to fragments of a known size to calculate the unknown fragment sizes. What is true of the DNA fragment band closest to the positive end of the gel?. Pour enough TBE buffer into the electrophoresis chamber so that it covers the gel. What do restriction enzymes do to the DNA? 2. Please read accompanying literature for your kit. Physiology Labs. The migration of immunoglobulins on agarose electrophoresis is evidence of their diversity. and one of gel electrophoresis. * Agarose Gel: Agarose Gel is a material which is used in biochemistry and biotechnology for gel electrophoresis and size rejected in chromatography; these are methods of organizing huge molecules by their size and electrical charge. The activity is completed with an electrophoresis to test for Huntington's disease in the patients. We provide a world to be explored rather than a path to be followed. Complete this worksheet as you walk through the steps of the labs. Lab: BIOL-006B-05 [CRN 00224] Protein electrophoresis 1: JAN 09: Cutting DNA 2: DNA gel electrophoresis S2i: Virtual DNA Digest: JAN 16:. The gel is already prepared. You will use polymerase chain reaction (PCR) and gel electrophoresis to perform genotyping in order to identify the gene that causes double muscling in cattle. Temperature Effect. In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. Evaluate amplified DNA by agarose gel electrophoresis followed by ethidium bromide staining. org/wgbh/nova/education/body/create-dna-fingerprint. Bacterial Transformation Lab moved to TODAY. These labs can be used as stand alone labs, as pre-labs for a classroom assignment or as a setting to. Further Information. Scientists can identify DNA segments by measuring how long they are using a technique called gel electrophoresis. – please show an “example calculation” (you may neatly hand-write this) ̵…. Lab 10 gel electrophoresisworksheet x electrophoresis 3 pages virtual homework assignment 1 doc. A Virtual Gel Electrophoresis Lab Worksheet can be used to help determine the chemical composition of an object. Substrate Concentration. Everything on the lab bench had to be on ice so the even distribution of heat and cold can be kept. Answer all questions in the lab notebook to complete the lab. Email: [email protected] DNA: draw and label a picture/model 2. · Gel electrophoresis chamber · Power supply · Staining tray · Semi-log graph paper · Ruler · Lab notebook Common Workstation · 0. Lab Operations: Biometrics. – MLT to MLS Overview. This process uses electricity to separate DNA fragments by size as they migrate through a gel matrix. What does a restriction enzyme do to DNA? 5. The activity is completed with an electrophoresis to test for Huntington's disease in the patients. Chapter 12 Dna Rna Answers Guided Reading Study Work, Guided Reading Activity 10. 23 - Evolution 1) Darwin's Evidence of Evolution 2) Hardy-Weinberg Theory 3) Genetic Drift. Teaching Philosophy Teaching Experience CHEM 111: Chemistry and the World CHEM 120: Chemistry of Life’s Beginnings CHEM 125: Structural Chemistry and Equilibrium CHEM 260: Medicinal Chemistry…. Gel Electrophoresis Drew Linarelli Honors Biology 17 May 2016 Period 9. During gel electrophoresis, an electrical current is applied to a gel mixture, which includes the samples of the DNA. I & II Mitosis Lab Experiment Aug. Once it has cooled the comb is removed. Due to the fact we wish to offer all you need in a single genuine as well as reputable supply, most people present valuable information about numerous subject matter and also topics. Nanotechnology. Pour a 60 ml 1% agarose/1X TAE gel keeping in mind that you will need at least 9 lanes. BioSurplus, Inc. Join us this week for murder and mayhem in the lab!. The acronym SDS-PAGE stands for sodium dodecyl sulfate – polyacrylamide gel electrophoresis. Let's begin by looking at how restriction enzymes work. The gel is stained with ethidium bromide so you can visualize how these DNA molecules resolved into bands along the gel. Computation of size of DNA and Protein Fragments from Their Electrophoretic Mobility (Reference: Raghava, G. Use the forward and back arrow to progress through the simulation. Students watch a video and answer 5 Multiple Choice Questions (can be HW) Exam Style Question and Mark Scheme. Sample buffer is 50 mM TrisCl, pH 7. Virtual DNA GEL electrophoresis. Joe Biology Transpiration Set-up * Biology Transpiration setup. Note that several of the more abundant protein bands, such as albumin, are. The protein electrophoresis test is often used to find abnormal substances called M proteins. Only DNA Samples should produce amplification (visible band as a Gel result, or high Fluorimeter value). 3 sh (may not be repeated for credit) Prerequisite: CHM 2210 The course is a first in a series of Clinical Chemistry courses for the Medical Laboratory Sciences student. Gel electrophoresis, one of the most common laboratory techniques in molecular biology, has a wide range of applications in the life sciences. Gel Electrophoresis Drew Linarelli Honors Biology 17 May 2016 Period 9. In the top left of the cover picture, a LIB with low remaining capacity as a result of ongoing capacity loss is shown. As such, we chose it as a platform to expose high school and undergraduate students to the active process of scientific inquiry in general, while specifically teaching electrophoresis. Gel electrophoresis is a separation technology that uses gel, a substrate (like gelatin), electricity (“electro”), and movement (“phoresis”, from the Greek verb phoros, meaning “to carry across”). Objective: Students will learn the steps of DNA fingerprinting by creating a fingerprint in a virtual lab. PAGE is a powerful analytical technique having numerous applications in modern biology. It has been discovered that Suspect 2 _____ the blood sample found on the sink at the house. Preview this quiz on Quizizz. Lab: Gel Electrophoresis Online Simulation On the bottom of the Gel Electrophoresis Virtual Lab click on “Can DNA Demand a Verdit” or the following link. It focuses on the principles of electrophoresis and Western Blotting of proteins to check for their expression patterns. Directions: For this mini-practical you are going to be doing two online simulations: one of PCR (polymerase chain reaction) and one of gel electrophoresis. Dissertation Abstract. Virtual Labs on Frontiers in Biochemistry. This is a sample from a known Neisseria meningitidis patient. Gel Electrophoresis Virtual Lab #1 (Colorado State University) Gel Electrophoresis Virtual Lab #2 (University of Utah) Drag and Drop Pedigrees DNA - The Double Helix (Noble Prize. Student Information Background Information: The Sanger sequencing method, which is also known as the dideoxychain termination method, produces a nested set of radioactive fragments from a template strand by replicating the template strand to be sequenced and interrupting the replication at one of the four bases. Related posts of "Virtual Gel Electrophoresis Lab Worksheet" Community Helpers Police Officer Worksheet Previous to discussing Community Helpers Police Officer Worksheet, make sure you are aware that Training can be all of our key to a more rewarding down the road, and also discovering doesn't just stop once the education bell rings. In side, there is a 10% starch solution. Virtual Lab: Animal Genetics Simulation Virtual Lab | Labster View Lab Report - DNA & Genes - Virtual Lab Test – BIOL 1408 from BIOL 1408 at South Texas College. (as a corollary, if the buffer's been sitting a while and evaporates such that the ionic concentration increases, too much current flows and you risk boiling it, which melts an agarose gel and destroys an acrylamide one). Polymerase Chain Reaction Virtual Lab posted Oct 3, 2012, 14. (scopes) https://n11099d39416. Unit: Genetics, Scientific Method, or Ecology (if done in conjunction with NYS Lab #1: Relationships and Biodiversity as a pre-lab activity) Objectives: Students will be able to…. To understand how the process works, one must first learn the gel electrophoresis definition. : AP ENVIRONMENTAL WEBLABS - UCA labs with good descriptions and great standard and simulation labs. This lab will introduce you to SDS-PAGE (sodium dodecyl sulfate - polyacrylamide gel electrophoresis), a simple and inexpensive method for resolving proteins in complex mixtures. Conclusion: Gel electrophoresis allows scientists to visualize the sizes of DNA segments and aids in the sequencing of lengths of DNA. In this lab students will model the procedure of setting up the gel, loading the DNA samples, assembling the gel apparatus, hooking up the electric current, and finally analyzing the results. Flash Player. Background: As the newest and least experienced student in a biochemistry research lab, you spend your first few weeks washing glassware and labeling test tubes. * Agarose Gel: Agarose Gel is a material which is used in biochemistry and biotechnology for gel electrophoresis and size rejected in chromatography; these are methods of organizing huge molecules by their size and electrical charge. summarize how gel electrophoresis is used to separate molecules. Define gel electrophoresis. In this lab you will mount a SDS-polyacrylamide gel, apply proteins chromatographically separated in the previous lab, and perform the electrophoresis. Electrophoresis · Virtual Lab: Agarose Gel Electrophoresis of Restriction Fragments – Colorado State University · Electrophoresis Simulation – Rochester Institute of Technology. Click on High School Manipulation > Techniques > sorting and sequencing. Resources: Unit 6 Gel Electrophoresis Lab Hands-on Lab. Extract human DNA and run it on a gel in the University of Utah's virtual laboratory. 1 DNA Electrophoresis Lab-CIBT Version AGAROSE GEL ELECTROPHORESIS LAB ACTIVITY AT A GLANCE Goal: To determine the presence or absence of DNA that has been amplified through PCR and to quantify the size (length of the DNA molecule) of the product. The lab is based on using gel electrophoresis for DNA fingerprinting. Which band pattern among Lanes 3 to 6 seems to be the closest match to the band pattern in Lane 2, where the crime-scene DNA fragment was loaded?. Next week we will actually conduct a lab where we use gel electrophoresis to identify a suspect. Complete questions 3 and 4 in the Beaks of Finches Lab. DNA RESTRICTION DIGEST AND GEL ELECTROPHORESIS: A VIRTUAL LAB by bcitstudents – Click here to launch virtual lab – (Screenshot #1) This is the virtual version of the UBC Advanced Molecular Biology Laboratory’s experimental kit #2 (see Restriction Digest of Lambda DNA and Gel Electrophoresis for details) which features a common and important molecular technique used in laboratories to. Unit: Genetics, Scientific Method, or Ecology (if done in conjunction with NYS Lab #1: Relationships and Biodiversity as a pre-lab activity) Objectives: Students will be able to…. To assemble, take out the gels from the casting frame and clamp them in the gel apparatus. Introduction. Pick a tool that would represent the beak of your Finch. Terasaki Life Sciences Bldg 4121. It is used to make lots of copies of DNA and is even used by the police to give a large enough quantity of DNA for testing from the smallest blood or other sample. Curley explains how to perform our gel elctrophoresis lab. P (LO) , IST‑1. Electrophoresis · Virtual Lab: Agarose Gel Electrophoresis of Restriction Fragments – Colorado State University · Electrophoresis Simulation – Rochester Institute of Technology. Sort and measure the DNA molecules in this virtual gel electrophoresis experiment. Enzymes Phosphatases Antarctic Phosphatase DNA polymerases Reverse Transcriptase Phusion Terminal Transferase Pfu T4 DNA Polymerase Pfx50 DNA ligases Thermostable DNA ligase RNA Polymerases Escherichia coli RNA polymerase Restriction enzymes EcoRI SpeI XbaI PstI XmaI DpnI NheI MfeI NsiI Other Lysozyme Proteases Proteinase K Electrophoresis Agarose gel electrophoresis Ethidium bromide TAE DNA. Increase Efficiency. Created by : mrcastiglione. Biotechnology. As such, we chose it as a platform to expose high school and undergraduate students to the active process of scientific inquiry in general, while specifically teaching electrophoresis. ” Polyacrylamide Gel Electrophoresis (Theory) : Molecular Biology Virtual Lab II : Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab. Gel electrophoresis simulation This simulation goes with the Living Environment lab "Agarose Gel Electrophoresis Lab Sheet". The size of the STRs at each genetic locus is determined using a genetic analyser. 16 - Gel electrophoresis lab, DNA practice activities (Activities 7-4, 7-5, 7-6) DRESS. How is Gel Electrophoresis Done? • The agarose gel is a solid jelly like substance to which the DNA mixture (with a dye) is added to • An electrical current is added to the gel and forces the pieces of DNA to move across the gel Summary of Gel Electrophoresis of DNA The Gel Results • In this technique the rate at which the different pieces of. In Gel Electrophoresis Proteins and nucleic acids are electrophoresed inside a matrix or "gel". Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size. Effect of Inhibitors on Enzyme Activity. The professionals we have work hard within the required tim e to deliver lab reports on time. The charged macromolecules migrate across a span of gel because they are placed in an electrical field. Pour a 60 ml 1% agarose/1X TAE gel keeping in mind that you will need at least 9 lanes. We provide a world to be explored rather than a path to be followed. We would like to show you a description here but the site won't allow us. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0. How many base pairs are there in the human genome? _____ 2. The lab is based on using gel electrophoresis for DNA fingerprinting. Questions: 1. Activity Summary Hands-on labs teaching students about biotechnology can be expensive and tedious for teachers to prepare. In our lesson, we discussed using gel electrophoresis for nanotechnology, specifically determining if the PEG molecule has been attached to the quantum dot. Today, we'll be talking about gel electrophoresis. The buffer conducts the current. 2 Below is a diagram of an electrophoresis chamber or "gel box" containing an agarose gel submerged in electrophoresis buffer (liquid that will conduct electrical current) as viewed from the side: DNA molecules have a net negative charge for reasons you will learn later on. Analyzing the food sample with DNA isolation, PCR amplification, and gel electrophoresis completes this activity. Metabolism. The DNA result was submitted to the CODIS DNA databank which forwarded the name John Doe as the suspect. Gel electrophoresis worksheet. The Amgen Biotech Experience is an international education program funded by the Amgen Foundation. When using the Gel Electrophoresis, we took notice of the number of fragments there were and. What is the material of the gel similar to. It is very "hands-on" even for an online resource, as students have to do a lot of clicking/dragging/dropping etc. 12 - Virtual PCR lab, virtual gel electrophoresis lab, web-based activities Feb. Additional funding and support are provided by LA Promise Fund, New England BioLabs, the Orange County Biotechnology Education Partnership, Pasadena Bioscience Collaborative, LA / OC Biotech Center, Fullerton College, Pierce College, and California State University - Channel Islands. Temperature Effect. Genetics 14 July 2009. Virtual Gel Electrophoresis Lab Worksheet having Beneficial Subjects. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut by a single restriction enzyme. – Electronic temperature control via dial, LED temperature display,over-temperature safety control, independent thermostat support– Chamber: 11. Students learn how fragments form unique patterns, which help to distinguish the base for DNA identification. Analyzing the food sample with DNA isolation, PCR amplification, and gel electrophoresis completes this activity. This lab will introduce you to SDS-PAGE (sodium dodecyl sulfate - polyacrylamide gel electrophoresis), a simple and inexpensive method for resolving proteins in complex mixtures. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the Classic edition of The Biology Place to all educators and their students. Then gel electrophoresis will determine the lengths of each DNA fragment by sending smaller fragments towards the positive charged side of the gel. It is designed to help students link chemical computations with authentic lab chemistry. DNA Fingerprinting. Purpose: This activity is meant to learn and understand how protein can be isolated from DNA fragments through gel electrophoresis technique. Gel Electrophoresis. Biotech Equipment & Labware. Sodium-Dodecyl Sulfate, the first part of this, or "SDS", is an anionic detergent. Even though this lab presents a different application for gel electrophoresis, the lab helps you to understand how. Gel Electrophoresis Simulation. SDS-PAGE stands for Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis. Virtual Lab Manager at HappiLabs, Recovering Acaddemic Podacst Co-host, Deputy Editor for The POSTDOCket Shoham Band Research assistant interested in biological science, patient education, and. Agarose Gel Electrophoresis of PCR products and RD reactions. Gel Electrophoresis – Virtual Lab Author: Matthew Nicastro Created Date: 3/2/2020 4:16:32 PM. Review, verify, and document exercise completion. Finally you are. Our crime laboratory is very much like a 'typical' police lab, except the victim is an animal. Click the Explore button to learn about the lab items available for your investigation. Because the sugar-phosphate backbone of DNA has a strong negative charge, the current drives the restriction fragments. The following experiments offer a series of interactive, inquiry-based biology simulations and exercises designed for college and AP high school biology students. Students will get hands-on experience using micropipettes and gel electrophoresis boxes while getting to visualize DNA on a gel. Virtual electrophotogram. Boss Level. And let's talk about how it works. After using the Electrophoresis to process some DNA, what was the estimated length, in base pairs, for each band in your DNA sample? (You will find this after you go through the electrophoresis virtual lab. DNA Learning Center resources are the best in scientific educational materials. Email: [email protected] Find out here how gel electrophoresis works :. 1 The addition of serum free light chains (sFLC) and IFE increases detection of MM to >99% and is consistent with the International Myeloma Working Group recommended guidelines. Log in to create and rate content, and to follow, bookmark, and share content with other members. 8% agarose solution (or gel, if prepared ahead of time) · 1 x TAE (tris-acetate-EDTA) buffer · Methylene blue stain Slide 14 / 26 Casting Agarose Gel Step 1 Seal the ends of the gel-casting tray with tape. Gel_Electrophoresis_Virtual_Lab (1) - Gel Electrophoresis Highlight your answers. The Virtual Lab is an online simulation of a chemistry lab. Gel Electrophoresis – Virtual Lab Name: Answer Key Date: Class: Gel Electrophoresis Virtual Lab Worksheet Instructions Go to the following link and complete the gel electrophoresis virtual lab: Select “Gel Electrophoresis” from the list and start the virtual lab. Teaching Philosophy Teaching Experience CHEM 111: Chemistry and the World CHEM 120: Chemistry of Life’s Beginnings CHEM 125: Structural Chemistry and Equilibrium CHEM 260: Medicinal Chemistry…. Create a DNA Fingerprint (NOVA) Nova Evolution Lab. Biotechnology Virtual Labs due Unit 6 Quiz (Ch 16-19) due on SchoolNet Friday 1/4 Chapter 20 reading due Tuesday 1/8 *ZERO HOUR* Lambda Gel Electrophoresis Day 1 Wednesday 1/9 Gel Lab Day 2 (staining day!) Friday 1/11 *ZERO HOUR* Unit 6 Test today! Wednesday 1/16 *ZERO HOUR* Quarter 2 Midterm Exam today! Friday 1/18 Lambda Gel Lab Report due. 5” W x 8” H x 19. Enzymes Phosphatases Antarctic Phosphatase DNA polymerases Reverse Transcriptase Phusion Terminal Transferase Pfu T4 DNA Polymerase Pfx50 DNA ligases Thermostable DNA ligase RNA Polymerases Escherichia coli RNA polymerase Restriction enzymes EcoRI SpeI XbaI PstI XmaI DpnI NheI MfeI NsiI Other Lysozyme Proteases Proteinase K Electrophoresis Agarose gel electrophoresis Ethidium bromide TAE DNA. Professional gel electrophoresis of DNA help. To analyze results and to deduce genotypes from given phenotype and pedigree information. Our mobile lab equipment inventory (including reagents) is made available to ‘Āina-Informatics Network teachers - this includes micropipettes, a PCR thermal cycler, gel electrophoresis equipment, a blue light transilluminator and more. It's easy to teach students the basics of DNA gel electrophoresis and analysis with this classic AP(r) Biology lab. 00, as of -0001-11-30. ” Polyacrylamide Gel Electrophoresis (Theory) : Molecular Biology Virtual Lab II : Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab. 1 The addition of serum free light chains (sFLC) and IFE increases detection of MM to >99% and is consistent with the International Myeloma Working Group recommended guidelines. "Gel Electrophoresis Virtual Lab. The DNA is visualised in the gel by addition of ethidium bromide, which is mutagenic, or less-toxic proprietary dyes such as GelRed, GelGreen, and SYBR. Image of a human DNA gel electrophoresis DNA gel electrophoresis. make sure you read. Even though this lab presents a different application for gel electrophoresis, the lab helps you to understand how this. Find gel electrophoresis lesson plans and teaching resources. C+: This stands for positive control. Ppt Biotechnology Genetic Engineering Powerpoint"> Full Template. On the gel picture below, (a) circle the smallest fragment produced by a restriction enzyme and label it “smallest. The gel image on the right is an SDS-PAGE gel of the same samples. Go through the simulation once without answering the questions, then go through a second time answering the questions on the worksheet. Also you should not open your amplicon tubes or do the gel electrophoresis in the same room as any of the places described above. Electrophoresis of DNA fragments on agarose or polyacrylamide gel and ethidium bromide staining. This separates the molecules of different sizes. Describe agarose: 7. Which band pattern among Lanes 3 to 6 seems to be the closest match to the band pattern in Lane 2, where the crime-scene DNA fragment was loaded?. Virtual Biology Labs: Virtual labs that allow students to save predictions, data, calculations and conclusions from lab (Adobe Flash Player required) Virtual Interactive Bacteriology Laboratory: Virtual labs with pre-lab walk through (descriptions, steps, starts) ( some make students do all or some steps). The general electrophoresis techniques. You need to upgrade your Flash Player. pGLO: a multi-day exploration. DNA: draw and label a picture/model 2. During the running and staining of your gel (steps 9 and 11), perform Part II of this lab, "Extraction of Mitochondria", which will be needed for next week's lab. Second edition. [email protected] This activity takes approximately 3 hours to complete with time for other activities between various steps. Watch this science video tutorial from Nurd Rage on how to make sodium silicate from drain cleaner and gel beads with Dr. Publications. Even though this lab presents a different. Providing large numbers of undergraduate students in scientific disciplines with engaging, authentic laboratory experiences is important, but challenging. In order to target proteins with MWs between 20 and 200 kDa, you will need to create a conventional SDS-PAGE gel using the recipes shown below. ) Alternatively, you could work through this virtual gel lab (This is a Flash site that won't work on most phones. You then rinse the gel repeatedly with water, so that the dye washes off the gel. The lab is based on using gel electrophoresis for DNA fingerprinting. Gel Electrophoresis Overview. Exercise 2 – Agarose gel electrophoresis of restriction digests 1. Agarose Gel Electrophoresis. For use with 96-well plates, including QIAamp 96 plates and RNeasy 96 plates. If the DNA from the two sources is the same, identical banding patterns should occur. Silberring, in Proteomic Profiling and Analytical Chemistry (Second Edition), 2016. Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size. You will use polymerase chain reaction (PCR) and gel electrophoresis to perform genotyping in order to identify the gene that causes double muscling in cattle. Read the description that appears for each item. edu-2020-05-02T00:00:00+00:01 Subject: Gel Electrophoresis Virtual Utah Lab Answer Key Keywords: gel, electrophoresis, virtual, utah, lab, answer, key Created Date: 5/2/2020 6:26:40 AM. This Journal of Biological Chemistry (JBC) Classic on using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine the molecular weight of proteins is one of our most highly cited articles. Pick a tool that would represent the beak of your Finch. Students will assess their own Per3 genotypes using PCR and gel electrophoresis, while also assessing their sleep phenotypes through a simple circadian questionnaire, with the ultimate goal of learning about circadian clocks and the. Setting up the SDS-PAGE Apparatus: Images from College of Charleston's Virtual Molecular Biology LabBook. Created by : mrcastiglione. Buy Mettler Toledo 11132535 Bluetooth Wireless Option for XS Balances and more from our comprehensive selection of Mettler Toledo Output / Relay Kits. Virtual Gel Electrophoresis Lab Worksheet having Beneficial Subjects. Gel Electrophoresis Virtual Lab. Biological Image Analysis Virtual Lab. Buy Electrophoresis Station - EL-100 Four Station, SB49186 at Nasco. AP Biology 12 Molecular Biology Lab 6B: Virtual Electrophoresis Learning Intentions o How does gel electrophoresis separate DNA fragments? o How can you use a standard curve to determine the size of unknown DNA fragments?. Topics to study for test: 1. Gel-based high throughput comparison between the protein expression profiles of normal and diseased tissues can be done in either of the two ways, viz. Dan was a graduate student from 2013 to 2018, and post-doc from 2018 to 2019 in the lab. The Virtual Lab is an online simulation of a chemistry lab. There are, however, some issues related to the different versions of Java and their compatibility with different versions of ImageJ. video of the dissection of a frog. The OpenPCR system successfully amplified products of the expected size. The lab is based on using gel electrophoresis for DNA fingerprinting. They will use this fingerprint to solve a virtual crime. In this interactive activity from the Dolan DNA Learning Center, learn about gel electrophoresis, the standard technique that makes comparative analysis of DNA samples possible. In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. What is the material of the gel similar to? A sponge made of jell-o 3. Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis. It can also stand in on its own as a virtual lab in cases where it is impossible for students to do a real-life PCR lab. Run the gel for 15 minutes. 2 Quiz Study Guide - QUIZ ON TUESDAY!!!. We will use it for analyzing gel electrophoresis experiments. Gel electrophoresis is an analysis method implemented in all disciplines of life sciences. The lab is based on using gel electrophoresis for DNA fingerprinting. Update effective for all #372 kits purchased after December 20, 2016. Gel electrophoresis worksheet. In this lab you will mount a SDS-polyacrylamide gel, apply proteins chromatographically separated in the previous lab, and perform the electrophoresis. Virtual Labs. [One 50 min class period for introduction, 1-3 class periods for research/diagnosis & tests reporting, 50 min gel electrophoresis, and partial class period follow-up and final analysis]. So first, you need to have the gel. This lab can be easily shared with students through Google Classroom or the use of a Google Drive. Click the Explore button to learn about the lab items available for your investigation. Resources: Unit 6 Gel Electrophoresis Lab Hands-on Lab. A method for DNA moleculs separation. biotechnology webquest - Free download as PDF File (. Biology Labs On-Line. What is electrophoresis? 10. A Hirt DNA Extraction is an isolation of all extrachromosomal DNA in a mammalian cell. and one of gel electrophoresis. Students learn how fragments form unique patterns, which help to distinguish the base for DNA identification. ” Bitesize Bio, 16 Feb. Gel Electrophoresis uses a gel and electricity to separate molecules for analysis. 4points Due In One Week Part 4 Csi Wildlife Usi"> Full Template. The VEG has become a popular device for the laboratory and its popularity is growing with the fact that it is a fully functional unit with additional features that were not available in the past. The gel is stained with ethidium bromide so you can visualize how these DNA molecules resolved into bands along the gel. Because the sugar-phosphate backbone of DNA has a strong negative charge, the current drives the restriction fragments. Virtual labs give teachers an inexpensive way to teach students about multiple scientific topics. · Gel electrophoresis chamber · Power supply · Staining tray · Semi-log graph paper · Ruler · Lab notebook Common Workstation · 0. Agarose Gel Electrophoresis of PCR products and RD reactions. Office: (310) 825-9093 Lab: (310) 825-3270. What is electrophoresis used for? To separate fragments based on size 2. Since these DNA fragments are too large to separate by conventional agarose gel electrophoresis, the use of an alternating voltage gradient system known as pulsed-field gel electrophoresis was developed. 5” W x 8” H x 19. Gel Electrophoresis Explore electrophoresis with The Amoeba Sisters! This biotechnology video introduces gel Virtual Labs 2019 Recap - PraxiLabs Virtual labs of Praxilabs had a fruitful year full of awards and achievements, University of California Press and in this short video you will go through our main. Global Warming Complexity. The OpenPCR system successfully amplified products of the expected size. Gel electrophoresis from the Genetics Science Learning Center at the University of Utah RNA lab from PBS Chemistry. 8% agarose solution (or gel, if prepared ahead of time) · 1 x TAE (tris-acetate-EDTA) buffer · Methylene blue stain Slide 14 / 26 Casting Agarose Gel Step 1 Seal the ends of the gel-casting tray with tape. Gel electrophoresis is a method that uses electricity to separate charged molecules (like DNA fragments) based on size, and this separation is carried out in a porous matrix called a gel (see lab manual). What is gel electrophoresis, you might ask. Increase Efficiency. Gel electrophoresis has been an integral part of molecular biology labs for decades, finding utility in analysis, separation, molecular engineering and clean-up of nucleic acids. Virtual Gel Electrophoresis Lab Worksheet – Powdered agarose is placed into a flask, followed by means of a salt water solution referred to as a buffer. agarose, polyacrylamide) consist. 2 (In Related Information, see profile 120256 Immunofixation (IFE), Serum, Protein Electrophoresis (PE), Serum, and Quantitative. This molecular technique can be used to isolate a gene of interest and to. Student Worksheet Virtual Lab. Update effective for all #372 kits purchased after December 20, 2016. The OpenPCR system successfully amplified products of the expected size. Polyacrylamide Gel Electrophoresis (Prior to lab, please review the lecture notes as well as Stryer on the topic of electrophoresis. 2 Products found. Microarray. 8) After electrophoresis, get your group's labeled stain box, be sure the box label matches your gel letter and half fill it with deionized water. Electrophoresis · Virtual Lab: Agarose Gel Electrophoresis of Restriction Fragments - Colorado State University · Electrophoresis Simulation - Rochester Institute of Technology. Education Center - K-12 Lessons and Laboratories - Classroom Activities in Plant Biotechnology: Activity 4 - Transformation of E. coli cells with the plasmid pGLO, which contains a gene for “green fluorescent protein.